In addition the increasing number of vendors supplying the leaves of this plant in any form via the internet has made the plant globally available as there is no restriction or legislation against possession green malay kratom high dose of this plant except in the source countries (Malaysia Thailand etc). Green Green Malay Kratom Powder apart from the effects of using this plant seen with traditional users and drug addicts as described previously in chapter 1(section 1. With the introduction of legislation against possession who has the best kratom online of this plant in Malaysia the access of this plant to the public especially to drug addicts is now under tighter control. Like many other traditional remedies that exist in the market the potential toxicity of this plant and its derivatives are not fully known. Based on the long use of this plant by humans with no reports on serious health effects or cancer formation it might be difference between bali thai kratom assumed that the use of this plant is safe. All substances are poisons; there is none that is not a poison. The hypothesis was tested using various in vitro techniques which assessed the cellular and biochemical consequences of exposure.
MIT has a lesser effect and cells arrest mainly at G1 phase in SH-SY5Y cells. The cell arrest occurring at high doses of MIT was found to be correlated with p53 and p21 expression although the expression changes were marginal Green Green Malay Kratom Powder compared to control and lower dose groups. The mechanism for cell cycle arrest in the cells treated with high doses of MSE remains unclear as there was no correlation with p53 and p21 as both proteins were lost after the treatment.
Human lymphoblastoid – MCL-5 cells For this cell line the cell cycle analysis was carried out using Cellquest Pro kratom 60x dosage software and the aggregated cells (doublet cells) were gated out. The DNA profiles were determined using Modfit LT cell cycle analysis software (Verity Software Topsham ME). The effect of MSE for 24 and 48 hr time period (Fig. M phase cells was noted for all doses compared to control cells for the first 24 hr treatment period.
Annu Rev Cell Dev Biol. The cell cycle: Principles of control. Oxford University Press.
M MIT-like compound. This is not dissimilar to the experimentally determined IC50 for pure MIT of 7. To assess the long-term effect of MSE on surviving cells after acute treatment a clonogenicity assay was performed after 24 hr treatment on HEK 293 and SHSY5Y cells.
The trypan blue assay and clonogenicity assay were employed as described in chapter 2 section 2. MSE and MIT are discussed as follows: Effects of naloxone on MSE and MIT treated cells: Fig. Naloxone also appears to successfully inhibit the MIT toxicity (Fig.
Interestingly for MIT there was a clear significant difference of caspases 3 and 7 activities at both concentrations of MIT tested. This kratom powder or capsules finding suggests that the mode of the cell death of MIT treated cells is dependant on caspase 3 and 7 activation pathway. There were no significant differences in the kratom king bali review subG1 population (apoptosis population) between treated groups (caspase 3 inhibitor caspase 8 inhibitor caspase 9 inhibitor and general caspase inhibitor treated with high dose of MSE) and the control and negative control groups. At this stage it seems that despite having high MIT content in the MSE the high dose MSE treatment in SH-SY5Y cells does not activate caspase enzymes. This probably could be due to other chemicals that present in MSE preventing the activation of caspase enzymes. Cell death of SH-SY5Y cells after MSE and MIT appeared to be predominantly via apoptosis based on its morphological appearance however biochemically the results discussed above fail to support a caspase mediating event. As apoptosis could Green Green Malay Kratom Powder
follow various pathways and often vary in different cells (Esposti and McLennan 1998 Hetts 1998) this prompted us to further investigate
if other pathways could contribute.